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Multiple Sequence Alignment using Clustalw

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Please input the sequences to be aligned in FASTA format

Pairwise alignment speed
Reset gap alignments



Note: Click here for information on the parameters

Pairwise Alignment Parameters

Slow/Accurate alignaments:
Gap open penalty
Gap extension penalty

Protein weight matrix :
BLOSUM30 PAM350 IDENTITY MATRIX

Fast/Approximate alignments:
Gap penalty
K-tuple (word) size
No. of top diagonals
Window size
Pairwise alignment speed

Multiple Alignment Parameters

Gap open penalty
Gap extension penality
Delay divergent sequences
Toggle Transitions (DNA)

Protein Weight Matrix :
BLOSUM series PAM350 IDENTITY MATRIX

Use negative Matrix

Format Of Alignment Output

CLUSTAL format output
NBRF/PIR format output
GCG/MSF format output
PHYLIP format output
GDE format output

Protein Gap Parameters

Toggle Residue-Specific Penalties
Toggle Hydrophilic Penalties
Hydrophilic Residues
Gap Separation Distance
Toggle End Gap Separation

Last updated
accesses since 5th June 1996.